U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R34
Horseradish Peroxidase
(color development solution)

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Solution A (horseradish)


HRP color development reagent 60 mg
Ice cold methanol 20 ml

Mix to dissolve. Protect from light and prepare fresh.



Solution B


Ice cold hydrogen peroxide, 30% 60 1
Tris-buffered saline 100 ml

Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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