U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R22
ELISA Buffer
(for Cholera Toxin Test)

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Bovine serum albumin 1.0 g
NaCl 8.0 g
KH2PO4 0.2 g
Na2HPO4 2.9 g
KCl 0.2 g
Distilled water 1 liter
Tween 20 0.5 ml

 

 

 

Adjust pH to 7.4 and add Tween 20. Store frozen. Thaw before use.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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