U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R19
Dulbecco's Phosphate-Buffered Saline (DPBS)

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NaCl 8.0 g
KCl 200 mg
Na2HPO4 1.15 g
KH2PO4 200 mg
CaCl2 100 mg
MgCl2· 6H2O 100 mg
Distilled Water 1 liter

 

 

 

Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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