U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M8
Alkaline Peptone Agar

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Peptone 10 g
NaCl 20 g
Agar 15 g
Distilled water 1 liter


Boil to dissolve ingredients. Adjust pH so that value after sterilization is 8.5 ± 0.2. Autoclave 15 min at 121°C. Solidify agar in tubes as slants.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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