U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M78
Letheen Agar (Modified)

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Letheen agar (Difco or BBL) 32 g
Trypticase peptone 5 g
Thiotone peptone 10 g
Yeast extract 2 g
NaCl 5 g
Sodium bisulfite 0.1 g
Agar 5 g
Distilled water 1 liter

 

 

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 x 100 mm petri dishes. Final pH, 7.2 ± 0.2.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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