M71
Kligler Iron Agar
|
M71
Kligler Iron Agar
| Polypeptone peptone | 20 g |
| Lactose | 20 g |
| Dextrose | 1 g |
| NaCl | 5 g |
| Ferric ammonium citrate | 0.5 g |
| Sodium thiosulfate | 0.5 g |
| Agar | 15 g |
| Phenol red | 0.025 g |
| Distilled water | 1 liter |
Heat with agitation to dissolve. Dispense into 13 x 100 mm screw-cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.
Commercially available from Difco, BBL, and Oxoid.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21
