U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M67
Irgasan-Ticarcillin-Chlorate (ITC) Broth

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Tryptone 10 g
Yeast extract 1 g
MgCl2·6H2O 60 g
NaCl 5 g
Potassium chlorate 1 g
Malachite green, 0.2% 5 ml
Distilled water 1 liter

 

 

 

Adjust to pH 7.6 ± 0.2 and autoclave at 121°C for 15 min.


 

Add the following:

Ticarcillin (1 mg/ml) 1 ml
Irgasan DP300 (1 mg/ml) 1 ml

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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