U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R63
Hugh-Leifson Glucose Broth (HLGB)

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Peptone 2 g
Yeast extract 0.5 g
NaCl 30 g
Dextrose 10 g
Bromcresol purple 0.015 g
Agar 3 g
Distilled water 1 liter

 

 

Heat with agitation to dissolve agar. Adjust pH to 7.4 ± 0.2. Autoclave 15 min at 121°C.

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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