U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M45
Duncan-Strong (DS) Sporulation Medium, Modified
(for C. perfringens)

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Proteose peptone 15 g
Yeast extract 4 g
Sodium thioglycollate 1 g
Na2HPO4.7H2O 10 g
Raffinose 4 g
Distilled water 1 liter

 

 

Dissolve ingredients and sterilize by autoclaving for 15 min at 121°C. Adjust to pH 7.8 ± 0.1, using filter-sterilized 0.66 M sodium carbonate.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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