U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M43
Cooked Meat Medium (Modified)

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(a) Cooked meat medium
           (commercially available in dehydrated form)


Beef heart 454 g
Proteose peptone 20 g
Dextrose 2 g
NaCl 5 g

(b) Diluent
            (not available commercially)


Tryptone 10 g
Sodium thioglycollate 1 g
Soluble starch 1 g
Dextrose 2 g
Neutral red (1% aqueous) 5 ml
Distilled water 1 liter

 

 

Adjust pH to 6.8 ± 0.2. Add 1 g dehydrated (a) and 15 ml (b) to 20 x 150 mm tubes. Let meat particles rehydrate. Autoclave 15 min at 121°C.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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