U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M4
Acid Broth

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Proteose peptone 5 g
Yeast extract 5 g
Dextrose 5 g
K2HPO4 4 g
Distilled water l liter

 

Dissolve ingredients and dispense 12-15 ml portions into 20 x 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 5.0.


 

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21