U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M36
Cell Growth Medium

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Mix the following sterile solutions aseptically:

Minimal essential medium
with Hanks' salts (MEMH)		 1 liter
L-15 medium (Leibovitz)
(containing 100 units penicillin G,
100 5g streptomycin, and
50 5g gentamicin per ml) 		1 liter

 

Mix on magnetic stirrer, filter through 0.20 µm membrane, and dispense into sterile 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.


 

Just before use add:

Fetal bovine serum 200 ml
NaHCO3 (7.5%)

50 ml

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21