U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M34
Casamino Acids-Yeast Extract-Salts Broth
(CA-YE)
(Gorbach)

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Casamino acids 20 g
Yeast extract 6 g
NaCl 2.5 g
K2HPO4 8.71 g
Trace salts solution
(below), optional		 1 ml
Distilled water 1 liter

 

 

Adjust pH so that value after autoclaving is 8.5 ± 0.2. Autoclave 15 min at 121°C.


 

Trace salts solution (optional)

MgSO4 50 g
MnCl2 5 g
FeCl2 5 g
Distilled water 1 liter

 

Suspend ingredients. Add enough 0.1 N H2SO4 to dissolve. Sterilize by filtration through 0.45 µm membrane. Add 1 ml to 1 liter base. Dispense 10 ml portions into 50 ml flasks.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21