M19
Bismuth Sulfite Agar
(Wilson and Blair)
|
M19
Bismuth Sulfite Agar
(Wilson and Blair)
| Polypeptone (or peptone) | 10 g |
| Beef extract | 5 g |
| Dextrose | 5 g |
| Na2HPO4 (anhydrous) | 4 g |
| FeSO4 (anhydrous) | 0.3 g |
| Bismuth sulfite (indicator) | 8 g |
| Brilliant green | 0.025 g |
| Agar | 20 g |
| Distilled water | 1 liter |
Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 ± 0.2
DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48 h.
| CAUTION | DO NOT AUTOCLAVE |
| PREPARATION | Day before use. |
| STORAGE | In Dark |
| Loss of Selectivity after 48 h | |
Hypertext updated by rim/cjm 2005-OCT-21
