U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M186
Trypicase (Tryptic) Soy Broth
with ferrous sulfate

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Trypticase peptone 17 g
Phytone peptone 3 g
NaCl 5 g
K2HPO4 2.5 g
Glucose 2.5 g
Ferrous sulfate 35 mg
Distilled water 1.0 liter

 

 

Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.



Hypertext Source: adapted from Chapter 5, Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Created 2000-MAR-01

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