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Bacteriological
Analytical
Manual
Online
January 2001
M147
Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar
| Yeast extract |
5 g |
Oxgall |
5 g |
| Peptone |
10 g |
NaCl |
10 g |
| Sucrose |
20 g |
Ferric citrate |
1 g |
| Sodium thiosulfate 5H2O |
10 g |
Bromthymol blue |
0.04 g |
| Sodium citrate 2H2O |
10 g |
Thymol blue |
0.04 g |
| Sodium cholate |
3 g |
Agar |
15 g |
| Distilled water |
1 liter |
Prepare in flask at least 3 times larger than required volume of medium. Add ingredients to warm distilled water and heat to dissolve. Bring just to boil, and immediately remove from heat. DO NOT AUTOCLAVE! Cool to 50°C and pour into petri dishes. Dry the plates overnight or at 37-45°C before use.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
