U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M14
Antibiotic Medium No. 1
(Agar Medium A)

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Gelatone or gelysate 6 g
Tryptone or trypticase 4 g
Yeast extract 3 g
Beef extract 1.5 g
Dextrose 1 g
Agar 15 g
Distilled water 1 liter

 

 

Autoclave 15 min at 121°C. Final pH, 6.5-6.6. Commercially available in dehydrated form as Difco Penassay Seed Agar or BBL Seed Agar.

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21