U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M136
Shigella Broth

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Base


Tryptone 20 g
K2HPO4 2 g
KH2PO4 2 g
NaCl 5 g
Glucose 1 g
Tween 80 1.5 ml
Distilled water 1 liter

 

 


Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.



Novobiocin solution

Weigh 50 mg novobiocin into 1 liter distilled water.

Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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