M104
MR-VP Broth
|
M104
MR-VP Broth
| Buffered peptone-water powder (Difco or BBL) |
7 g |
| Glucose | 5 g |
| K2HP04 | 5 g |
| Distilled water | 1 liter |
| Pancreatic digest of casein | 3.5 g |
| Peptic digest of animal tissue | 3.5 g |
| Dextrose | 5.0 g |
| Potassium phosphate | 5.0 g |
| Distilled water | 1 liter |
Dissolve ingredients in water with gentle heat if necessary. Dispense 10 ml into 16 x 150 mm test tubes and autoclave 15 min at 118-121°C. Final pH, 6.9 ± 0.2.
| Peptone | 5.0 g |
| Glucose | 5.0 g |
| Phosphate buffer | 5.0 g |
| Distilled water | 1 liter |
Dissolve ingredients in water. Dispense 10 ml into 16 x 150 mm test tubes and autoclave 15 min at 121°C. Final pH, 7.5 ± 0.2.
For Salmonella: Dispense 10 ml into 16 x 150 mm test tubes and autoclave 12-15 min at 121°C.
For use with halophilic Vibrio spp., add NaCl to a final concentration
of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21
