U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R49
North Aniline (Oil)-Methylene Blue Stain

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Aniline oil 3 ml
Ethanol (95%) 10 ml
HCl (concentrated) 1.5 ml
Methylene blue (saturated solution) 30 ml
Distilled water to make 100 ml

 

Mix aniline oil with ethanol. Slowly add HCl with constant agitation. Add saturated methylene blue solution, dilute to 100 ml with water, and filter.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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