U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M87
Lysine Decarboxylase Broth
(Falkow)
(for Salmonella)

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Gelysate or peptone 5 g
Yeast extract 3 g
Glucose 1 g
L-Lysine 5 g
Bromcresol purple 0.02 g
Distilled water 1 liter

 

Heat until dissolved. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2.


 

For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21