Boil to dissolve peptone, phosphate, and agar in 1 liter of water. Add water to make original volume. Dispense in 100 or 200 ml portions and autoclave 15 min at not over 121°C. Final pH, 7.1 ± 0.2.

Before use, melt, and to each 100 ml portion add:

1. 5 ml sterile 20% lactose solution;
2. 2 ml aqueous 2% eosin Y solution; and
3. 4.3 ml 0.15% aqueous methylene blue solution.

When using complete dehydrated product, boil to dissolve all ingredients in 1 liter water. Dispense in 100 or 200 ml portions and autoclave 15 min at 121°C. Final pH, 7.1 ± 0.2.

Hypertext updated by rim/cjm 2005-OCT-21