U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M66
Indole Nitrite Medium
(Tryptic Nitrate)

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Trypticase 20 g
Na2HPO4 2 g
Dextrose 1 g
KNO3 1 g
Agar 1 g
Distilled water 1 liter

 

Heat with agitation to dissolve ingredients. Mix and dispense 11 ml portions into 16 x 150 mm tubes. Autoclave 15 min at 118°C. Final pH, 7.2 ± 0.2.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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