U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M49
EC Broth

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Trypticase or tryptose 20 g
Bile salts No. 3 1.5 g
Lactose 5 g
K2HPO4 4 g
KH2PO4 1.5 g
NaCl 5 g
Distilled water 1 liter

 

 

Distribute 8 ml portions to 16 x 150 mm test tubes containing inverted 10 x 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.9 ± 0.2.


 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21