U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M21
Blood Agar Base (Infusion Agar)

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Heart muscle, infusion from 375 g
Thiotone 10 g
NaCl 5 g
Agar 15 g
Distilled water 1 liter

 

Heat gently to dissolve. Autoclave 20 min at 121°C. Final pH, 7.3 ± 0.2. Commercially available dehydrated heart infusion agars may be used.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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