U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M162
Tryptone Phosphate (TP) Broth
(for enteropathogenic Escherichia coli)

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Tryptone 20 g
K2HPO4 2 g
KH2PO4 2 g
NaCl 5 g
Polysorbate 80 (Tween 80) 1.5 ml
Distilled water 1 liter

 

 

Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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