U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2002

M156
Trypticase Soy Broth Modified
(mTSB)

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Trypticase soy broth 30.0 g
Bile salts No. 3 1.5 g
Dipotassium phosphate 1.5 g
Novobiocin solution (R50) 0.2 ml
Deionized water 1 liter

 


Autoclave mTSB without novobiocin and let cool to room temperature. Final pH, 7.4 +/- 0.2. For chapter 24, gene probe method, add novobiocin just before adding food.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Revisions: January 28, 2002 Required pH specified.

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Hypertext updated by rim/kwg/cjm 2005-OCT-21