4. Reproductive/ developmental effects data needs

• Epidemiology for germ cell toxicity

• Expression profiling for genotoxic effects on somatic and germ cells

• Define the germ cell mutations generated

  • Dose-response
    • Low dose and multiple dose exposure effects
    • Compare food and drinking water exposures
  • Mechanistic
    • Adduct formation with DNA and significant nuclear proteins
    • Dominant lethal study in CYP2E1 knockout mice